Journal: Cardiovascular Diabetology

Article Title: The SGLT-2 inhibitor empagliflozin improves myocardial strain, reduces cardiac fibrosis and pro-inflammatory cytokines in non-diabetic mice treated with doxorubicin

doi: 10.1186/s12933-021-01346-y

Figure Lengend Snippet: Cardiac fibrosis and apoptosis in mice treated with saline solution (Sham), EMPA 10 mg/kg/day, DOXO 2.17 mg/kg/day or EMPA associated to DOXO (n = 6 for each group). A representative images of the interstitial fibrosis (collagen content, indicative of the fibrosis) in cardiac tissue. B cardiac collagen quantification (% of collagen of area) in heart tissue. C Heart pro-collagen 1-α1 quantification in cardiac tissue (ng/mg of total protein); D MMP-9 content ( pg/mg of protein) in cardiac tissue, indicative of fibrosis. E apoptosis imaging in cardiac tissue ( in green, apoptotic nuclei). F apoptotic nuclei, expressed as relative percentage of positive nuclei in heart tissue (two-way ANOVA with a Bonferroni post hoc test). G Caspase-3 expression in cardiac tissue (expressed as ng of Caspase-3/g of tissue). H SGLT-2 protein expression in cardiac and renal tissues of C57B/6 mice through western blot analysis. I Real Time -PCR analysis indicating the relative mRNA expression of SGLT-2 in cardiac and renal tissues of C57B/6 mice. Values are expressed ± SD. *** P < 0.001; **P < 0.01; * P < 0.05; ns: not significant

Article Snippet: Therefore, we quantified MMP-9 in heart tissues through Mouse MMP9 ELISA Kit (ab253227, AbCam, Milan, Italy) following the manufacturer’s instructions; results are expressed as pg of MMP-9/mg of protein (determined through Bradford assay).

Techniques: Imaging, Expressing, Western Blot, Real-time Polymerase Chain Reaction

Journal:

Article Title: Acute Humanin Therapy Attenuates Myocardial Ischemia and Reperfusion Injury in Mice

doi: 10.1161/ATVBAHA.110.205997

Figure Lengend Snippet: HNG improves cardiomyocyte survival in vitro and decreases apoptosis in response to Daunorubicin. Effect of HNG on A) Cardiomyocyte survival as assessed by cell viability assay, * p < 0.05 Dauno compared to Dauno+HNG, and B) early markers of apoptosis as assessed by CaspASE FITC -VAD-FMK in situ apoptosis assay.

Article Snippet: Effect of HNG on cardiomyocyte survival and apoptosis in vitro Rat heart myocardium cell line H9c2 (2-1) (cat# CRL-1446) was purchased from ATCC.

Techniques: In Vitro, Viability Assay, In Situ, Apoptosis Assay

Journal:

Article Title: Essential Oils as Components of a Diet-Based Approach to Management of Helicobacter Infection

doi: 10.1128/AAC.47.10.3240-3246.2003

Figure Lengend Snippet: Sensitivities of H. pylori strains to essential oils a

Article Snippet: The H. pylori strains used in the study were H. pylori P1 ( 2 ), isolated from a patient with nonulcer dyspepsia (reference strain); H. pylori Ly4, isolated from a patient with a family history of gastric cancer; H. pylori 1172 (ampicillin and metronidazole resistant); H. pylori 2234 ( cag negative); H. pylori 2322.7 ( cag positive); H. pylori ATCC 43504; and two mouse-adapted strains, H. pylori P49 (H. Kleanthous, T. Tibbits, T. J. Bakios, K. Georgopoulos, G. Myers, T. H. Ermak, J.

Techniques: